抗EspA单克隆抗体制备和纯化应用传统杂交瘤技术制备抗EspA的单克隆抗体,用饱和硫酸铵(saturated ammonium sulfate,SAS)沉淀法和HiTrap rProtein A柱纯化单抗。 2.
基于12个网页-相关网页
用饱和硫酸铵 saturated ammonium sulfate
Rabbit IgG were deposited by saturated ammonium sulfate, then IgG were purified with A-50.
饱和硫酸铵分级沉淀所得的兔IgG，经粗步纯化后再用A-50进一步纯化。
参考来源 - 猪干扰素α、β、γ基因克隆、原核表达及其单克隆抗体制备的研究Recombinant Treponema pallidum antigen TpN17 was expressed in pichia pastoris by methanol induction, then the samples was precipitated with saturated ammonium sulfate and further purified using a cation exchange column.
通过甲醇诱导，毕赤酵母表达系统中表达重组梅毒螺旋体抗原TpN17，样品经饱和硫酸铵沉淀粗提后，以阳离子层析交换柱对表达的TpN17进行进一步纯化。
参考来源 - 重组梅毒螺旋体抗原TpN17的纯化及基于金磁微粒梅毒螺旋体抗体的检测·2,447,543篇论文数据，部分数据来源于NoteExpress
沉淀抗原—抗体复合物的饱和硫酸铵最终饱和度为37.5％。
The antigen-antibody complex here was precipitated with saturated ammonium sulfate solution in a final saturation 37.5%.
球蛋白在半饱和硫酸铵溶液中即可析出，而清蛋白需在饱和硫酸铵溶液中才能析出。
The precipitation is egg albumin. We can take little precipitation (about matchhead size) in 1ml of distilled water, observe whether it is dissolved, identify by biuret reaction.
采用分步连续沉淀酶方法，确定硫酸铵沉淀饱和百分数范围。
This paper presents a multiple step and continuous precipitation experiment method to determine the accurate saturation percentages of ammonium sulphate precipitating enzymes.
应用推荐