在最严重的情况下，颈外动脉，肩带肌肉的颈部，迷走神经项，舌下神经号和舌分行的三叉神经也删除。
In most severe cases, the external carotid artery, strap muscles of the neck, Vagus nerve, Hypoglossal nerve and the lingual branch of the Trigeminal nerve are also removed.
第8天，除正常组外其余小鼠麻醉后，结扎双侧颈总动脉和迷走神经，造成小鼠急性脑缺血模型。
On the 8th day mice except in normal group were anesthetized and ligated bilateral common carotid artery and pneumogastric nerve so as to establish models of acute ischemia.
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