目的从微生物代谢物中筛选中性鞘磷脂酶抑制剂。
OBJECTIVE to screen neutral sphingomyelinase inhibitors from the metabolites of microorganism.
用CO2激光辐射筛选培育出高产鞘磷脂酶的工程菌——雅致放射毛霉变异株AE255-6。
High sphingomyelin phosphodiesterase-producing Actinomucor elegans mutant(AE255-6) was obtained by radiation of CO2 laser.
方法分离猪血细胞，用溶血，脱水，干燥等方法提取鞘磷脂，并用磷脂酶C降解鞘磷脂成神经酰胺。
METHOD Sphingomyelin was prepared through the process of separating erythrocyte from blood, erythrocytolysis, dehydration, dry, et al, and then hydrolyzed to ceramide by phospholipase C.
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