依据非辐射能量转移机理，测定了不同温度下溴甲酚紫与血清白蛋白相互结合时，其给体-受体间距离和能量转移效率。
According to the non-radiation energy transfer theory, the distances and energy transfer efficiencies between dye and protein at various temperatures were obtained.
结果：确定两化合物与牛血清蛋白的猝灭机理主要为静态猝灭和非辐射能量转移。
RESULTS The quenching mechanism of them were suggested as static quenching and nonradiative energy transferring.
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