这些酶在限制性位点(restriction sites)把DNA链切开，每种酶对应的限制性位点不一样。对于每一种酶，每个DNA分子可能有多个限制性位点，此时可以按照需要来选择切开某几...
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其间的差异来自少数限制性位点的突变。
The differences between haplotypes came from several dots mutation.
在研究突变机制时，发现有6例由于核苷酸的替换引起了限制性位点的增减。
In the study of mutation mechanism, it was found in 6 cases that there were gains and losses of sites due to nucleotide substitution.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
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