目的比较滴定法和紫外分光光度(U V)法在测定附子总生物碱中差异,探讨适宜附子总生物碱的含量测定方法。
Objective To analyze the differences in the determination of total alkaloids from Radix Aconiti Lateralis Preparata by Titration and UV, and investigate the suitable determination method.
方法:采用酸性染料比色法测定附子总生物碱含量,反相高效液相色谱法测定双酯型生物碱含量,蒽酮-硫酸法测定多糖含量。
METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively.
目的分析附子理中缓释片中总生物碱的药效物质。
Objective To obtain effective materials from the total alkaloids of Fuzi Lizhong sustained-release tablets.
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