方法传代得到单个HERG-HEK细胞,应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线(激活、失活、复活和去活化)。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
制作:将虾剪去钳、须、脚,洗尽沥去水。
Production: the shrimp clamp cut should be, foot to the waters Lek.
方法应用双电极电压钳技术分别在带滤泡膜及去滤泡膜后注入RNA并经孵育的卵母细胞记录膜电流。
Methods Dual-electrode voltage clamp technique was used to record membrane currents from follicular oocytes, and defolliculated oocytes that had been injected with alien RNA and incubated.
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