重组蛋白的产生是应用了重组DNA或重组RNA的技术从而获得的蛋白质。体外重组蛋白的生产主要包括四大系统:原核蛋白表达,哺乳动物细胞蛋白表达,酵母蛋白表达及昆虫细胞蛋白表达。生产的蛋白在活性和应用方法方面均有所不同。根据自身的下游运用选择合适的蛋白表达系统,提高表达成功率。
Inclusion bodies are aggregation which is formed during over-expression of recombination proteins. Such recombination proteins don’t have biological activities.
包涵体是重组蛋白在菌体内过表达后形成的聚集体,但是其所含的重组蛋白没有生物学活性。
参考来源 - 重组人核糖核酸酶抑制因子的复性及纯化The purification of recombination protein and the immunization of mice were undertaken and the antibody was obtained.
并进行了重组蛋白的纯化和免疫获得了相应的抗体。
参考来源 - P49抗原基因及Caenorhabditis elegans homologus基因在旋毛虫中的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
重组蛋白以包涵体的形式进行表达。
Expressed fusion proteins existed in the form of inclusion body.
瘦素, 抗肥胖蛋白(AOBP), 绵羊重组蛋白。
Leptin, Anti-Obesity Protein (AOBP), Sheep recombinant protein.
表达产物用亲和层析一步法进行纯化,然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
Like we talked about with insulin; make large quantities of this recombinant viral protein, and use that as a vaccine.
就像我们讲过的胰岛素,生成大量重组的病毒蛋白,并将其作为疫苗使用
应用推荐