然后对扩增产物进行限制性酶切反应；

Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.

youdao

方法：采用DNA测序及限制性酶切反应方法。

Methods:DNA sequencing and restriction endonuclease reaction was used.

youdao

方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。

METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.

youdao

Restriction enzymes are just a kind of enzyme, enzymes are protein molecules that make a chemical reaction go faster, and the chemical reaction that restriction enzymes do is cutting DNA.

限制性内切酶是一种酶,而酶是加速化学反应的蛋白质分子,限制性内切酶加速的化学反应,是切割DNA

耶鲁公开课 - 生物医学工程探索课程节选