目的研究胃泌素对人结肠癌细胞信号分子黏着斑激酶(FAK)酪氨酸磷酸化和蛋白质表达的影响。
Objective the study investigated the effect of gastrin on tyrosine phosphorylation and protein expression of focal adhesion kinase (FAK) in human colon cancer cells.
采用免疫沉淀及增强化学发光法检测IRS 1蛋白质酪氨酸磷酸化；
The tyrosine phosphorylation of IRS-1 was measured by immunoprecipitation and enhanced chemiluminescent immunoblotting technique.
鉴定了分子量为130000酪氨酸磷酸化的蛋白质为JAK2，一种非受体型酪氨酸激酶。
The tyrosine phosphorylated 130000 protein was identified as JAK2, a non receptor tyrosine kinase.
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