方法用建立的犬复合扩增体系进行PCR反应,用abi310型遗传分析仪对扩增产物进行检测。
Method the canine multiplex system were used for PCR reaction and the product were analyzed by ABI310 genetic analyzer.
结论:采用荧光标记引物进行复合扩增,结合PE310遗传分析仪自动分析,可以有效地区分各位点的扩增产物。
Conclusion: By co amplification using fluorescently labeled STR primers and detecting using PE310 genetic analyzer, the PCR products of these nine loci can be significantly distinguished.
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