目的探索松质骨骨基质明胶(BMG)作为软骨细胞培养支架的可行性。
Objective To investigate the feasibility of cancellous BMG as scaffold for chondrocyte.
目的:采用改良分步消化法进行软骨细胞培养,以获取大量纯净的软骨细胞。
OBJECTIVE: To obtain highly purified and large amount of chondrocytes by improved stepwise digestion method.
结果证实,采用改良分步消化法进行软骨细胞培养,在缩短了消化时间的同时其细胞生长及形态变化均无改变,可以顺利获取大量纯净的软骨细胞。
After 48 hours, the cells began to adhere. The results demonstrated that the improved method shortens digestion time, and easy to obtain a great amount of purified chondrocytes.
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