用酶切和PCR的方法对重组质粒进行鉴定。

Used restrict enzyme and PCR to verify the reconstructed plasmid.

youdao

结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。

Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.

youdao

结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。

Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

youdao

Or you could ask the question, I have this restriction enzyme, at what regions on this plasmid will it cut?

你也可以这样问,我的这种限制性内切酶,会从质粒的哪个区域切开呢

耶鲁公开课 - 生物医学工程探索课程节选

If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

耶鲁公开课 - 生物医学工程探索课程节选