笔者应用机械分离获得豚鼠单个初级精母细胞,采用全细胞式膜片钳技术记录该细胞离子通道电流。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
方法:在去除滤泡膜的非洲爪蟾卵母细胞上,建立双电极电压钳记录技术,并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。
Methods: the two-electrode voltage clamp recording technique was used to observe the ligand-gated and voltage-gated ion channel currents expressed in Xenopus laevis oocytes without follicles.
理论上,某种离子通道病变的患者,可以使用作用于该通道的制剂物治疗。
In theory, some ion channel diseases of the patients with a role in the drug treatment agent channel.
And actually, a question that might come up, I just explained, the sodium channel, you might say, well, how do potassium channels work then, because I can understand how you can filter something big out, but how do you filter out something small.
实际上,可能大家会问一个问题,我刚解释过,钠离子通道,你可以说,好吧,那么钾离子通道该如何起作用呢,因为我可以理解你是如何把大东西过滤掉的,但是你如何把小东西过滤掉呢?
应用推荐