网络释义专业释义
...达基因、利用PCR技术克隆同一家族的同源基因或利用酵母体系克隆新基因也是有效的途径；近年来，真核表达克隆(Expression cloning)的应用范围已被大大拓展，通过基因文库的随机大规模测序和EST的同源比较寻找和克隆免疫新分子更是大显身手，卓有成效。
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... Cloning and expression o 甘油单二酰酯脂肪酶基因的克隆和表达 cloning by expression 表达克隆 Cloning and expression 克隆表达 ...
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我们大约已经构建了20,000多个在3′端带有不同标签蛋白序列的全长ORF OmicsLink™ 表达克隆（Expression Clones）.你只要付一小笔额外的费用，我们可以除去终止密码子并把它克隆到你所需要的载体。
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方法 构建雌激素受体反义表达质粒 (pCASER) ,转染MCF 7细胞株 ,经G418筛选 ,PCR、Westernblot鉴定筛选出阳性表达克隆 (MTASER)。运用MTT法检测药物对细胞生长状况的影响。
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双语例句原声例句
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①目的构建重组蜂毒肽的原核表达克隆。
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结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
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用新霉素类似物(G418)筛选出阳性克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
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Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.
将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达
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We're going to have DNA that we would like to make lots of copies of, or we're going to have DNA that we would like to get expressed in a cell, and we're going to put it in this region that's called the polylinker.
我们将那些需要得到很多拷贝数的DNA,或者需要在细胞中表达的DNA,整合到这个叫做多克隆位点的区域中
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