分阶段测定血浆游离血红蛋白浓度，计算和比较转流后溶血指数(HI)。
Haemolytic Index (HI) was calculated and compared after circulation according to the values of free haemoglobin concentrations measured periodically.
方法：血浆标本与血红蛋白溶液混合后，其中的一氧化碳与血红蛋白结合，再用双波长分光光度法进行测定。
Methods:Mixing up plasma specimens and hemoglobin solution, the carbon monoxide in plasma is trapped with hemoglobin and then be estimated by a two-wavelength spectrophotometry.
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