蛋白分离的缓冲液系统(Laemmli buffer system Laemmli)比较 解离/非解离 native 缓冲液系统 很多应用蛋白质聚丙烯酰胺凝胶区带电泳(zone electrophoresis)的研究使用一种缓冲液系统,...
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结果显示:利用碱性硼酸钠缓冲液系统及一个改进的毛细管冲洗程序分离麦醇溶蛋白,可获得较高的分辨度和重复性。
The results showed that, higher resolution and reproducibility of gliadin HPCE separations were obtained by using the alkaline sodium borate buffer system and an improved capillary cleaning procedure.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
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