而后，按照荧光素的传统合成方法设计合成了五种氯代荧光素衍生物。
Thirdly, the five chlorofluorescein derivatives were designed and obtained by the traditional synthetic method of fluorescein.
为了改善荧光素的性能，通过对其结构进行修饰，开发研制出许多的荧光素衍生物，这些衍生物作为荧光试剂，更有利于对生物体进行检测。
To improve fluorescein's performance, a wide variety of fluorescein derivatives have been prepared by structural modification and used as fluorescent reagents favorable for detecting the organism.
香豆素及其衍生物作为生色团广泛应用于荧光探针分子的设计中，然而在水溶液中识别阴离子的香豆素类荧光探针鲜有报道。
Coumarins and its analogues have been widely used as chromophore in design of fluorescent probe, while less coumarin-based fluorescent probe was reported for detection of anion in water.
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