重组菌经培养,利用荧光显微镜检测重组酵母的荧光强度,然后通过荧光分光光度计检测分选酶与底物相互作用后产生游离的EGFP荧光强度进而确定分选酶的酶活。
After cultivation, recombinant cells were verified with fluorescence microscopy and sortase activity was detected by fluorescence spectrophotometer from variety of free EGFP's fluorescence intensity.
组分3的荧光强度与组分1荧光强度显著负相关;
The fluorescence intensity of component 3 was negatively correlated with that of component 1.
荧光强度与激发光强度的三次方关系表明,有效的荧光产生起源于三阶非线性光学效应的增强。
The intensity dependence of integrated luminescence on pump power shows that this is a third-order nonlinear optical effect.
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