GUS活性检测和胭脂碱分析表明,外源基因在转化细胞内得到稳定表达。
Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli.
其中以开花初期的植株,利用胭脂碱型农杆菌介导(如c58),真空渗入5分钟的处理方法转化效率最高。
It was concluded that using the nopaline type of Agrobacterium (e. g. C58), 5min vacuum infiltration, and using the plants with just several blooming flowers would give the best results.
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