结果：证实本实验方法避免了假阳性，背底十分清亮。

Results: The improved method avoided false positive reactivity, and a clear background was observed.

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发现P物质样阳性胞体主要位于PAG的中、尾段，分为位于腹外侧区的腹侧群和位于腹外侧区与背外侧区交界处的外侧群等两群。

SP-Like positive cells were mainly located in the middle and caudal segments of the PAG, these were divided into two groups, ventral and lateral groups.

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PAS染色及FGF7、10免疫组化染色可见前肠背侧半及食管为阴性， 前肠腹侧半及气管部分为阳性；

The dorsal part of the common foregut and the normal esophagus were negative for PAS. FGF7, 10 were expressed on the places where the PAS staining were positive.

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