Also LAB-SA immunohistochemistry staining was adopted to detect and localize the expression of COX-2 protein in the whole-mounts of the intestine. And for the first time, transmission electron microscope (TEM) was used to investigate the changes in the intestinal muscularis.
并采用LAB-SA免疫组化染色法了解COX-2蛋白的表达及其在肠肌层中的细胞定位,本课题也首次使用透射电子显微镜观察移植肠肌层的变化,以期了解早期移植肠运动功能障碍的机理。
参考来源 - 大鼠原位小肠移植术后早期肠运动功能障碍的机理研究·2,447,543篇论文数据,部分数据来源于NoteExpress
肠黏膜表面显示有早期坏死,从黏膜向黏膜下层和血管壁肌层都有充血。
The mucosal surface of the bowel seen here shows early necrosis with hyperemia extending all the way from mucosa to submucosal and muscular wall vessels.
NADPH黄递酶组化法和计算机图像分析系统对两组肠黏膜肌层一氧化氮合酶(NOS)阳性神经纤维作定量分析;
The nerve fibers containing NOS in mucosal muscular layer of colon was quantitatively measured with NADPH diaphorase histochemistry assay and computer image analysis.
光镜下观察肠神经丛的分布,肠壁肌层sy免疫活性纤维的分布。
SY-immunoreactive nerve fibers in the bowel layers were graded under optical microscope.
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