目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。
Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.
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