维持培养液中适当低的氮水平，并保证足够量的磷供应即较低的氮磷比是促进塔玛亚历山大藻生长的重要原因。
Maintenance of continues low level nitrate with sufficient supply of phosphate, namely low N:P ratio, may facilitate the growth of Alexandrium tamarense.
为维持细胞在培养过程中的未分化状态，需要采用饲养层细胞培养，同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。
Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken es in an undifferentiated state.
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