使用液相色谱法分析电离能力比较强的样品时，样品在反相色谱柱上的保留时间很短或者根本不保留，这时要加入相应的离子对试剂，将分析物上的离子进行结合，形成在柱子上有保留的分子。我们使用离子对试剂将它留住。
把离子对试剂种类、浓度和丙酮、环己烷含量作为影响分离效果的因素。
The types and the concentrations of different kinds of ion pair reagents, the contents of acetone and cyclohexane were considered as factors which affected the results of separation.
以庚烷磺酸钠为离子对试剂，建立了同时测定头发中尼古丁和可天宁含量的反相离子对高效液相色谱方法。
A reversed phase ion-pair high performance liquid chromatographic method was developed for the simultaneous determination of nicotine and cotinine in human hair.
应用手性和非手性柱固定相，在正相色谱系统中，手性药物和手性离子对试剂形成非对映离子对复合物，从而使手性药物得以分离。
Separations based on formation of diastereomeric ion-pairs were fulfilled in systems with an organic mobile phase using achiral and chiral stationary phases.
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