ion exchange electrophoresis
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用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
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