将氯化钙,RNA(或DNA)和磷酸缓冲液混合,沉淀形成包含DNA且极小的不溶的磷酸钙颗粒。
方法:用磷酸钙共沉淀的方法向血管成纤维细胞转染MKP 1的基因;
Methods: MKP 1 gene was transfected into vascular fibroblasts by the classical calcium phosphate coprecipitation technique.
方法:人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
本研究认为鱼精蛋白改性磷酸钙是一种比共沉淀法合成磷酸钙更好的转染载体。
It could be expected that nucleoprotamine modified calcium phosphate could be a better transfect agent as compared to coprecipitated superphosphate.
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