方法用碱性磷酸酶-抗碱性磷酸酶（APAAP）免疫酶标和DNA末端原位标记（ISEL）双重染色方法检测38例急性白血病诱导化疗前后骨髓涂片中细胞凋亡程度及细胞类型，初治组17例与2 1例经治病例对照。
Methods A double-stained way of APAAP and in situ DNA end labeling (ISEL) were used to detect apoptosis characteristics in 38 cases of acute leukemia, including 17 untreated and 21 treated patients.
方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
P - TEFb是一个普通的转录因子，它通过磷酸化rna聚合酶ii (RNAP II) C -末端尾巴结构域(CTD)来调节转录延伸。
P-TEFb is a general transcription factor that regulates transcription elongation through phosphorylation of the C-terminal tail domain (CTD) of RNA polymerase II (RNAP II).
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