第三也是最有力的限制在于婴儿做出的是面对刺激的反应，还是从受到刺激到没受到刺激的变化，这是无法确认的。

The third, and most potent limitation is that it is not possible to be certain that the infant's response was due to the stimulus presented or to a change from no stimulus to a stimulus.

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通过脊髓液或者血液标本培养出细菌，做凝集试验或者聚合酶链反应(PCR)实验，会支持或确认诊断结果。

The diagnosis is supported or confirmed by growing the bacteria from specimens of spinal fluid or blood, by agglutination tests or by polymerase chain reaction (PCR).

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在送往开罗NAMRU 3海军医学研究所参考实验室的38份血液样本中，有13份经聚合酶链反应试验检测黄热病确认为阳性。

Of 38 blood samples sent to the reference laboratory, Naval Medical Research Unit, NAMRU 3 in Cairo, 13 were confirmed positive for yellow fever by PCR tests.

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