变性条件下通过Ni-NTA金属螯合亲和层析对表达产物进行纯化后再经梯度透析进行复性,采用破骨细胞样细胞(osteoclast-like cell,OLC)诱导分化实验来检测重组蛋白的生物活性,证实单核/巨噬细胞集落刺激因子(M-CSF)和破骨细胞分化因子...
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目的研究不同诱导因子对大鼠破骨细胞样细胞体外形成的影响。
Objective To investigate the effect of different osteotropic hormones on the formation of rat osteoclast like cell in vitro.
目的：探讨可行的获取人破骨细胞样细胞以供进一步研究的方法。
Object: the study is to find a feasibility method of obtaining human osteoclast-like cells for the purpose of further research.
目的：探讨伴破骨细胞样巨细胞乳腺癌的临床病理特点。
Purpose To study the clinicopathological features of mammary carcinoma with osteoclast-like giant cells.
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