当检测阀门数量较少时，例如大的柴油机，手动直接接触法可以使用。
When testing smaller Numbers of valves, e. g. large diesels, manual testing can be carried out in the direct contact mode.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
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