目的：制备轮状病毒外壳蛋白vp4。
1986年以来，利用病毒外壳蛋白及其它基因转化植物，获得具抗病毒能力的植株，已有大量成功的报道。
Since 1986, a lot of papers have been published on development of virus resistant plants transformed with genes from viruses, such as coat protein gene.
免疫胶体金标记显示，病毒外壳蛋白和病害特异蛋白存在于叶绿体、细胞质和细胞核以及细胞壁上，但线粒体中没有发现。
The coat proteins and the disease specific protein were found in the chloroplast, cytoplasm and nuclear, but not in mitochondria.
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