当细胞置于非常高的电场中，细胞膜就变得具有通透性，能让外界的分子扩散进细胞内，这一现象称为电融合，又叫电穿孔。
misc. electro-fusion
二倍体马铃薯体细胞电融合的研究 关键词：马铃薯；二倍体；电融合 [gap=1009]Key words: potato; dihaploid; electrofusion
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The effects of electrofusion protocol,activation method and embryo culture media on the development of interspecies reconstructed embryos were examined.
另外,本试验还比较了不同的电融合程序、激活方法和胚胎培养液对异种重构胚发育的影响。
参考来源 - 盘羊—绵羊异种核移植技术程序的优化研究Effect of different electro-fusion parameters on in vitro development of reconstructed embryo.
5.探讨了不同电融合参数对重构胚体外发育的影响。
参考来源 - 体细胞克隆小鼠胚胎的试验研究In order to obtain the high two cell fusion frequency in mass somatic fusion experiments, theoptimum electric paramete and other effective factors in potato electrofusion were studied .
但是目前在马铃薯的体细胞电融合具体操作中，普遍存在成对融合频率低的问题。
参考来源 - 提高马铃薯体细胞电融合成对融合频率的技术研究The embryos reconstructed by RNT could develop to blastocyst. But the cleavage rate of and blastocyst rate of reconstructed embryos were significant lower than that of electric fusion nuclear transfer (54.0% vs 75.1%, P<0.01\ 6.9% vs 15.9%, P<0.05).3.
结果显示,反向核移植可支持重构胚发育到囊胚,但其构建重构胚的效率极显著低于电融合法(54.0%比75.1%,P<0.01),囊胚发育率也显著低于电融合法(6.9%比15.9%,P<0.05)。
参考来源 - 水牛细胞核移植技术的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
同时讨论了电融合方法和结果。
实验三研究了不同浓度的电融合液对激活的影响。
The third experiment is about the concentration of the pulsating medium.
比较了微束激光融合法与病毒法，PEG法，电融合法。
Compares the method of cell fusion induced by laser microbeam with induced by virus, PEG, electric field pulse.
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