小麦TaMlo8基因的克隆及初步功能验证-毕业论文参考网 关键词：电子克隆；mlo8；荧光定量PCR；染色体定位；小麦 [gap=1583]KEY WORDS: In silico cloning；o8；FQ-PCR；Chromosome Location；heat
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7电子克隆(electronic cloning) 电子克隆.义称计算机杂交(computer hybridiza- tion),是以数学算法为手段,以计算机和互联网为T 具,利用现有的基因序列、表达序列...
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4、采用电子克隆(e-PCR)及反转录PCR(RT-PCR),从秦川牛脂肪组织中克隆并鉴定了牛ApoA4基因cDNA的编码区,并用生物信息学方法对ApoA4蛋白进行了预测分析,发...
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【摘要】 电子克隆(Cloning in silico)是指利用计算机技术,依托现有白勺网络资源,如EST数据库、基因组数据库等,采用生物信息学方法(同源检索、聚类、序列拼接等)延伸已...
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In recent years, the bioinformatics has becoming one of the focuses in Genetics and Biology. In silico cloning the novel genes based on the EST database is an important aspect in the field.
生物信息学是当前生物科学的研究热点之一，利用EST数据库电子克隆新基因的研究就是其中一项重要的内容。
参考来源 - 蜜蜂（Apis mellifera）ant基因和vha16基因的克隆及其功能预测的生物信息学研究In the process of insilico cloning, we found there was a piece of sequence had not been sequenced in genome of Apis mellifera, and then it was supplemented by sequencing.
测序结果表明,AmTpi序列与电子克隆序列完全一致。 在进行拼接比对过程中还发现AmTpi所在的蜜蜂基因组中含有一段未测出的序列(Gap),通过PCR产物测序将该段未知序列补充完整。
参考来源 - 蜜蜂磷酸甘油醛异构酶基因（Tpi）的电子克隆与研究The main research works and conclusions are as follows:(1) The first complete coding sequence (CDS) of CRHR1 and CRHR2 are obtained using the approach of combining homology and electronic cloning.
本研究的具体内容及结论如下:1、利用同源克隆与电子克隆结合的方法首次从猪脑组织中克隆得到CRHR1、CRHR2基因的全长编码序列(CDS)。
参考来源 - 猪CRHR1、CRHR2基因的克隆及mRNA表达规律的研究In this research, Based on in silicon cloning and bioinformatics analysis, Super hybrid rice parental lines Zhu 1 S was used as material, after RT-PCR and T-A clone, a 2219bp Zhu 1 S TIR1 full-length cDNA sequencec was obtained.
本文在电子克隆和生物信息学分析的基础上,设计特异引物,以超级杂交稻亲本株1S为材料,通过RT-PCR扩增并经T-A克隆后测序,获得一条长度为2219bp株1S TIR1的全长cDNA。
参考来源 - 超级杂交稻亲本株1S TIR1 cDNA的克隆与生物信息学分析·2,447,543篇论文数据，部分数据来源于NoteExpress
结论使用电子克隆技术对于发现新基因有重要的指导意义。
Conclusion Electronic cloning technology can be instrumental in identifying novel genes.
介绍了水稻电子克隆的基本原理、应用实例、相关的生物信息资源、存在问题和展望。
The principle, application, related resource of bioinformatics, problems and the prospect of in silico cloning of functional genes in rice were reviewed.
在鸡的大规模cDNA克隆测序的背景下，利用ESY数据库比对，应用电子克隆技术来寻找鸡的末知新基因。
Based on sequence alignment in EST database, new chicken genes were looked for by in silico cloning.
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