目的了解1，2 二氯乙烷对非洲绿猴肾细胞的损伤及其机理。

Objective To understand the injury mechanism of Vero cells (the kidney cells of the African green monkey) exposed to 1,2 Dichloroethane (DCE).

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方法根据SV40 - 776株设计合成三对引物对现有毒株进行PCR扩增并克隆测序。用这三对引物对56份猴肾、肺、脾及10批脊髓灰质炎疫苗进行检测。

MethodsKidneys, lungs and spleens of 56 monkeys and 10 poliovirus vaccines were examined by PCR using designed three pairs of primers on SV40-776 sequence.

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ACE2主要分布在恒河猴和人肾组织近端肾小管上皮细胞的管腔膜和胞浆。

ACE2 protein was mainly located in epithelium of proximal tubules of Rhesus monkey and human kidney.

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So, you would grow these monkey kidney cells in culture, you would add some virus to the culture broth, the cells would become infected, the virus would go through its life cycle.

我们在培养基中培养这些猴肾细胞,然偶加入一些脊髓灰质炎病毒,这样细胞就会被感染,病毒开始不断繁殖

耶鲁公开课 - 生物医学工程探索课程节选