SV40即猿猴病毒40(simian virus 40),是DNA肿瘤病毒的原型代表,其基因结构为共价闭合环状双股DNA分子,标准参考株SV40-776含5243个核甘酸,不同分离株bp数略有差异.
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从怀疑感染了猴痘病毒的人和动物身上采集的标本,应当由在装备适当的实验室工作并且经过培训的人员进行操作处理。
Samples taken from people and animals with suspected monkeypox virus infection should be handled by trained staff working in suitably equipped laboratories.
对该病毒的自然史仍存有疑问,需要进一步继续研究,以确定猴痘病毒的确切贮主,以及该病毒在自然界是如何生存下来的。
Doubts persist on the natural history of the virus and further studies are needed to identify the exact reservoir of the monkeypox virus and how it is maintained in nature.
尽管这样命名,猴痘病毒被认为主要寄居在啮齿动物内,如松鼠和巨型袋鼠。
Despite its name, monkeypox virus is believed to reside mainly in rodents such as squirrels and giant pouched rats.
They found that certain cells, in particular, epithelial cells from monkey kidneys, were very effective at propagating the virus.
他们发现有些细胞,尤其是在猴肾脏的上皮细胞中,这种病毒能够有效地增殖
So, you would grow these monkey kidney cells in culture, you would add some virus to the culture broth, the cells would become infected, the virus would go through its life cycle.
我们在培养基中培养这些猴肾细胞,然偶加入一些脊髓灰质炎病毒,这样细胞就会被感染,病毒开始不断繁殖
They grew the virus in monkey cell cultures, they purified the virus because you got to get all the other stuff from the cells that you're growing it in a way, they inactivated it by treating it with formalin which is just a formula-- it's just a mixture of Formaldehyde; Formaldehyde cross links proteins.
研究人员将病毒种植在猴细胞内,进行培养,并提纯病毒,在某种程度上,通过这种方法,可以得到所有类型的病毒,研究人员用福尔马林,处理病毒,使其灭活,福尔马林就是甲醛的水溶液,甲醛可以使蛋白质发生交联反应
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