然后诱导表达，用SDS - PAGE及蛋白印迹进行鉴定，然后进行蛋白纯化。
Then it was induced to expression and identified by SDS-PAGE and Western-blotting, and it was purified.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
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