方法用淋巴细胞分层法和低渗溶血法提取PBMC。

Methods Peripheral blood mononuclear cells(PBMC) were collected by using lymphocyte Ficoll and the low-osmotic hemolysis method.

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改良酶消化法和刮取法收集人输卵管上皮细胞，采用溶血法及差速贴壁法纯化上皮细胞。

Epithelial cells were collected with a modified method which included enzymatic method and scraping, and then the collected cells were purified by hemolysis method and differential adhesion method.

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目的探讨不同静脉采血方法对血液标本溶血现象的影响，并总结真空采血法的护理要点及注意事项。

Objective To explore the impact of different methods in blood drawing on sample hemolysis, and to summarize the nursing points of vacuum blood drawing.

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