超声波的引入,不仅大大提高了低温加碱的溶胞效果,而且对溶胞过程温度的要求有所降低。
The introduction of ultrasonic wave not only greatly improved the effect of lysis, but also decreased the requirements for the temperature during the lysis.
进一步提供了可选择的额外的纯化步骤,包括溶胞产物过滤、阴离子交换层析、三联体亲和层析和疏水性相互作用层析。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
在某些方法中,将所述溶胞产物在一定温度下加热一定时间,所述温度和时间足以使所述溶胞产物中存在的蛋白质变性并使其聚集和沉淀。
In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation.
They're small molecules, they're lipid soluble, they can go from extracellular to intracellular.
它们是小分子,并且是脂溶性的,它们能从胞外进入胞内
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