目的对荧光偏振免疫法(FPIA)测定血浆同型半胱氨酸(HCY)的方法学进行初步评价。
Objective To evaluate the detection of plasma homocysteine (HCY) with fluorescence polarization immunoassay (FPIA) technology.
方法:以1,6-二苯基,1,3,5-己三烯(DPH)作为荧光探针,利用荧光偏振法测定脾细胞膜脂质区的流动性。
METHODS: The fluidity of membrane lipid regions of splenocytes was determined by the fluorescence polarization technique using 1, 6-diphenyl-1, 3, 5-heatriene (DPH) as a fluorescence probe.
经过分析,该装置不仅能完成偏振光的干涉实验,而且可以测定晶体的折射率或折射率分布情况。
These devices can not only accomplish polarized light interference experiment, but also measure the refractivity or refractive index profile of crystal.
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