目的：研究雷洛昔芬对血管内皮细胞雌激素应答元件(ERE)转录活性的影响与雌激素受体(er)亚型的关系。
Objective: to investigate the effect of Raloxifene on the estrogen responsive element (ERE) transcription activity and its relationship with estrogen receptor subtypes in vascular endothelial cells.
启动子是基因表达调控的重要元件，启动子的活性间接反映了它所控制的基因的表达。
Promoters are important elements that regulated gene expression, activity of promoters indirectly reflect gene expression that they control.
以含雌激素反应元件的荧光素酶(ERE - LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。
The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).
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