该技术的缺点是：必须保证油不会从物镜组的小室中泄漏出去。

The downside of this technology was insuring that the oil would not leak out of the lens cell.

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对显微镜物镜浸油与媒质水溶液折射率不同所产生的球差对光阱力的影响进行了定量计算与数值分析。

Effects of spherical aberration caused by the refractive mismatch between objective oil and medium water on optical trapping force is calculated quantitatively and also analyzed.

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