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洗脱物经预柱脱盐和浓缩后进入毛细管反相柱进行反相分离，分离后的肽段直接进入质谱仪离子源进行一级和二级质谱的分析。
Following desalinisation the eluted peptides were further purified by reversed-phase capillary column(150 mm 75 m) and online analysed by the coupled tandem mass spectrometry.
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