毕赤酵母表达(pichia pastoris expression )实验手册一.毕赤酵母表达常用溶液及缓冲液的配制二.毕赤酵母表达的培养基配制三.主要试验环节的操作3.1 酵母菌株的分离纯化...
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Using the Pichia pastoris expression system, theβandγsubunits of Arabidopsis G protein were successfully expressed.
利用巴斯德毕赤酵母表达体系成功表达了拟南芥异三聚体G蛋白的β和γ亚基。
参考来源 - 拟南芥异三聚体G蛋白β和γ亚基在毕赤酵母中的表达及β亚基和RACK1相互作用研究·2,447,543篇论文数据,部分数据来源于NoteExpress
毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
目的:利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白,为进一步研究LG1-3的结构和功能间的关系奠定基础。
Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.
结论:为下一步在毕赤酵母中组成型表达外源蛋白,研究其作用机理和遗传机制奠定了基础。
Conclusion:The GAP promoter can be used to express foreign protein effectively and is the basis for studying the physicochemical properties and genetic mechanism of Gassericin T.
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