FISH和RFLP方法比较费时费力且花费昂贵，而特异性P CR标记又在一定程度上受模板DNA浓度的影响。

FISH and RFLP are the most Labour intensive, time consuming and more expensive methods. The special PCR markers, in some degree, are effected by the content of template DNA.

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在比较原始的环境中，例如在许多热带地区普遍存在的那类环境，水稻种植是一种非常费力费时的手工作业。

Under more primitive circumstances, such as those that prevail in many of the tropical lands, rice growing is an exceedingly laborious and time-consuming hand operation.

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