次代细胞培养:将原代细胞培养物轻微消化后,
经6次传代培养后,其生物量和菌丝多糖产量较稳定,且明显超过原始菌株,表明其遗传稳定性良好。
After continuous cultivation for 6 generations, the biology yield and the polysaccharide yield were steady and obviously exceeded the original strain. It showed a good genetic stability.
经3~ 4次继代培养即可得到悬浮的单细胞。
Suspension single cells were obtained after successive subculturing of 3 or 4 generations.
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