(新页面: {{BBAHeader}} ;模拟表位(Mimotope): A '''mimotope''' is a macromolecule, often a peptide, which mimics the structure of an epitope.
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Up to now, many hemagglutinin-neuraminidase (HN) nucleotide sequences of NDV strains were obtained, but there is still few reports about its mimotope.
目前已获得多株NDV血凝素—神经氨酸酶(HN)的基因核苷酸序列,但对其抗原模拟表位的研究还鲜有报道。
参考来源 - 新城疫病毒血凝素—神经氨酸酶模拟表位及其免疫生物学特性研究·2,447,543篇论文数据,部分数据来源于NoteExpress
在以模拟表位肽为基础肽苗设计中,MAP结构是一种较好的结构。
MAP structure is a good candidate to design a mimic peptide-based vaccines.
方法1。根据HSP70全长基因序列设计针对其编码区的引物,在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
将化学合成的模拟表位肽与载体BSA偶联后,以之建立无毒素的竞争酶联免疫检测方法,并与常规酶联免疫吸附检测方法及高效液相色谱比较。
A competitive ELISA was set up with a mimotope peptide conjugated to BSA carrier, and the new method was compared with conventional ELISA method and HPLC method.
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