研究了酶转化芦丁产物异槲皮甙的分离提纯。
We have separated and purified the isoquercitrin from rutin by enzyme reaction.
通过高效液相色谱检测纯度,结果表明异槲皮甙转化率为70%,纯度为90%以上。
Determined the purity by HPLC, it was found that the converting rate of isoquercitrin from rutin was about 70%, and the purity was more than 90%.
利用RP-HPLC法,采用保留时间定性,结合化学方法,证实罗汉果中含有黄酮甙元山奈酚,并首次发现含有黄酮甙元槲皮素,第一次总结出罗汉果鲜果中总黄酮含量的变化规律。
A kind of flavonol named quercetin was first found and by their retention times in RP-HPLC and chemical characters a known flavonol named kaempferol was proved to be contained in Luohanguo fruits.
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