结论： 桥粒芯糖蛋白1+2在皮肤鲍温病中表达部分下调，可能与肿瘤的恶性程度有关。
Conclusion: The expression of desmoglein 1+2 is partly down regulated in Bowen disease, and may be related to the high and low malignancy of tumor.
目的探讨副肿瘤性天疱疮（PNP）患者血清中抗桥粒芯蛋白（DSG）抗体与头皮脱发之间的关系。
Objective To investigate the relationship between the levels of antidesmoglein(DSG)1, 3 antibodies in the sera of patients with paraneoplastic pemphigus(PNP)and alopecia.
目的：扩增人表皮角质形成细胞桥粒芯糖蛋白4胞外区域EC 1, EC 2, EC3和EC4的核酸序列。
AIM To amplify the nucleotide sequence of desmoglein 4 extracellular domains EC1 EC2, EC3 and EC4 from human keratinocytes.
克隆人桥粒芯胶蛋白3前导肽(Dc3p)基因，并进行表达、纯化和鉴定。方法：用PCR法从桥粒芯胶蛋白(Dc)cDNA文库中扩增出Dc3p基因，与pGEX-4T-2载体连接成重组表达质粒pGEX-4T-2/Dc3p；重组质粒转入B121大肠杆菌：经诱导表达谷胱甘肽S-转移酶-Dc3p(GST-De3p)融合蛋白，该蛋白经GlutathioneSepharose4B亲和层析柱纯化，SDS-PAGE和免疫印迹法检测鉴定。结果：经酶切及序列分析鉴定，重组质粒pGEX-4T-2/De3p成功构建，诱导后高表达GST-De3p融合蛋白并得到纯化，免疫印迹法证实表达蛋白为Gsr-Dc3p。结论：获得高表达、高纯度的GST-Dc3p融合蛋白，为De3p的功能及免疫学分析打下基础。
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