重组菌经培养，利用荧光显微镜检测重组酵母的荧光强度，然后通过荧光分光光度计检测分选酶与底物相互作用后产生游离的EGFP荧光强度进而确定分选酶的酶活。
After cultivation, recombinant cells were verified with fluorescence microscopy and sortase activity was detected by fluorescence spectrophotometer from variety of free EGFP's fluorescence intensity.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
评估神经细胞变化的指标检测：采用显微荧光分光光度计测定细胞老化指标脂褐素水平（相对荧光值）反映细胞老化程度；
The evaluation of nerve cell aging index: The level of lipofuscin (relative fluorescent value) index of cells aging was detected by micro-spectrofluorometer to reflect the degree of cell aging;
应用推荐